Tissue Dissociation Enzyme Neutral Protease Assessment

Clinical islet isolation outcomes with a highly purified neutral protease for pancreas dissociation

BACKGROUND Pancreas dissociation is a critical initial component of the islet isolation procedure and introduces high variability based on factors including the enzyme type, specificity and potency. Product refinement and alterations to the application strategies have improved isolation outcomes over time; however, islet utilization from donor organs remains low. In this study we evaluate a low...

Collagenases for Tissue Dissociation

[Activation mechanism of calcium-activated neutral protease].

The activation mechanism through limited autolysis of a calcium-activated neutral protease (CANP) with a high sensitivity to calcium ions (pCANP) was analyzed. The rate of autolysis was dependent on pCANP concentration. The reaction was inhibited by high concentrations of digestible substrates but not by a nondigestible substrate. Incubation of pCANP inactivated by Nethylmaleimide with a small ...

Improving Colorant Absorption from Pistachio Hulls on Wool Fiber Using Protease Enzyme

Nowadays, textile processing based on biotechnology has gained its importance in the view of stringent environmental and industrial safety conditions. The use of protease enzymes in protein fibers to improve some physical and mechanical properties is particularly interesting. In this research, wool yarns were first treated with different concentrations of protease enzymes in aqueous solution in...

Purified Tissue Dissociation Enzymes: Past, Present, Future Challenges

The incorporation of purified neutral protease and class I & class II collagenase isolated from C. histolyticum culture supernatants into defined tissue dissociation enzyme (TDE) mixtures was in part responsible for the success of the Edmonton protocol. The enzyme composition of Liberase HI Purified Enzyme Blend (HI) was determined by using design of experiment methodology to optimize the conce...